Coding

Part:BBa_K248001:Design

Designed by: Marina Badia Fabregat   Group: iGEM09_UAB-Barcelona   (2009-10-16)

nsrR (nitrite-sensitive transcription repressor)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 285
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 285
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 285
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 285
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is not compatible with the Biobrick standard assembly 10 because there is an EcoR I restriction site inside the encoding region.


Source

PCR from Nitrosomonas europaea (ATCC 19718).

References

Beaumont, H.J.E., Lens, S.I., Reijnders, W.N.M., Westerhoff, H.V., Spanning, R.J.M. (2004) Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite-sensitive transcription repressor. Molec Microbiol 54:148-158